Search:
Applications
FAQs
Manuals
Literature Library
Metrohm Support
Get Support
Subscriptions
 
Enter your postal code:
 
 
Applications
On-line
 

We are committed to providing you with up-to-date and useful applications support. Click below to access applications information online.

 
Practical Information for the molecular biologist Go to top
Conversions
Nucleic acid conversions
Protein conversions
Spectrophotometric conversions
Abbreviations, symbols, and units
The genetic code
Properties of acids and bases
Radioisotopes
Detection of nucleic acids and proteins
Calculating the required field strength for electroporation
PCR
Calculating primer quantity
Molecular structure of the amino acids
Cell Technology Go to top
Microdissection
Piezo-power microdissection: two examples of its applications
  1. BioTissue Engineering –The isolation of living cartilage cells 
  2. Cancer Research – Isolating cell areas for human lung carcinoma from cryosections
 (PDF 256k)
Cancer Research – Isolating cell areas for human lung carcinoma from cryosections (PDF 1.1M)
The isolation of cellular aggregates (PDF 1.4M)
The isolation of living cartilage cells (chondrocytes) (PDF 316k)
Microinjection/Manipulation
Microinjection of plasmid DNA or double stranded RNA into the gonads of C. elegans (PDF 720k)
Microinjection of plasmid DNA constructs for rapid expression in mammalian cells (PDF 124k)
Microinjection of DNA, RNA and tracer dyes into early fish embryos (PDF 220k)
Sample preparation for microinjection (PDF 532k)
Transgenic animals (PDF 1.2M)
Intracytoplasmic Sperm Injection (ICSI)-Procedure and Equipment (PDF 256k)
Centrifugation Go to top
The influence of the centrifugation temperature on urological carcinoma cell lines (PDF184k)
Software controlled acceleration and braking ramp for optimized isolation of mononuclear cells  (PDF 144k)
Consumables Go to top
Decontamination and inhibition of ribonucleases  
Eppendorf PerfectPure C-18 tip: A reproducible method to reversibly bind, purify, and concentrate peptides for increased MS spectra quality (PDF 216k)
General technical data and application notes for LoBind microcentrifuge tubes (PDF 420k)
Eppendorf Micro Test Tubes—The best material for original Eppis! (PDF 160k)
Eppendorf® Biopur—A unique dimension in biological purity (PDF 84k)
Chemical stability of disposables (PDF 384k)
Filter tips (PDF 248k)
Detection – DNA, RNA, and Protein Quantification Go to top
Consumables
Analysis of leptin receptor expression (OB-R L) in fat and muscle tissure using the UV-transparent plastic cuvette UVette for the photometric quantification of RNA (PDF 308k)
Easy preparation of total RNA from cultured cells with TRIZOL® and Phase Lock Gel (PDF 256k)
Preparation of DNA from mouse tail with Phase Lock Gel (PDF 252k)
Sources of errors with photometric measurement of nucleic acids (PDF 76k)
Expand your spectrum—UVette adapters for UVette (PDF 512k)
UVette® material compatibility chart
The new generation of cuvettes—UVette (PDF 80k)
The new UVette—superior performance (PDF 56k)
Microbiological turbidimetry using standard photometers (PDF 804k)
Turbidity measurements (OD600) with abdorption spectrometers (PDF 364k)
Rabbit antibody repertoire as a novel source for the generation of therapeutic human antibodies (PDF 848k)
Instruments
BioPhotometer—Quantification made easy (PDF 60k)
UVette—The ideal cuvette for measurings in the UV range (PDF 148k)
Electrofusion Go to top
Protocols for Eppendorf Multiporator®
Guide for generating hybridomas by electrofusion (PDF 924k)
Bovine oocytes and fetal fibroblasts (PDF 104k)
Human lymphocytes (blood) + CB-F7  (PDF 80k)
Human lymphocytes (blood) + H73C11 (PDF 320k)
Human lymphocytes (blood) + SHM-D33  (PDF 80k)
Human lymphocytes (tissue) + H73C11 (PDF 308k)
Lipid Vesicles (PDF 500k)
Mouse lymphocytes + SP2/0 AG 14 (PDF 316k)
Mouse oocytes  (PDF 208k)
Tetraploid mouse embryos (CD 1) (PDF 60k)
Tetraploid mouse embryos (CD 1, B6D2, C57, C57/CBA) (PDF 56k)
Production of tetraploid mouse embryos by electrofusion (PDF 84k)
Yeast protoplasts (PDF 252k)
Electroporation Go to top
Genetic reporter systems
Efficient transfection of neurospora crassa
Principles and applications of electrical cell fusion (PDF 170k)
General protocol – cell lines (PDF 80k)
Optimization protocol – cell lines  (PDF 84k)
Protocols
Protocols for Eppendorf Electroporator 2510
Protocols for Eppendorf Multiporator
Optimizing Electroporation Parameters
Laboratory Automation Go to top
epMotion® 5070
Determination of Pancreatic Elastase 1 on epMotion 5070 or 5075 (PDF 408k)
Automated real-time PCR set-up with the epMotion 5070 in comparison to the manual set-up (PDF 252k)
Successful qPCR with small reaction volumes on Eppendorf Mastercycler ep realplex (PDF 644k)
Automated PCR setup in the 384-well format without cross-contamination with the liquid handling workstation epMotion  5070 (PDF 312k)
High-throughput, fully automated real-time PCR diagnostics of HBV and salmonella (PDF 248k)
PCR product purification using the Eppendorf epMotion 5070 liquid handling workstation together with the Eppendorf Perfectprep PCR Cleanup 96 kit (PDF 164k)
Precision and accuracy to the epMotion system (PDF 104k)
Contamination test for automated dispensing system - epMotion 5070 and 5075 (PDF 128k)
Minimization of remaining volumes in plates and tubes (PDF 64k)
Facilitating PCR setup via an automated liquid handling system (PDF 360k)
Loading the Invitrogen® E-Gels with the epMotion 5070 (PDF 228k)
epMotion  5075
Use of robotic systems to enhance performance of real-time PCR for detection of nine major respiratory viruses (PDF 124k)
Determination of Pancreatic Elastase 1 on epMotion 5070 or 5075 (PDF 408k)
Automated PCR of buccal-swab FTA cards in a 96-well format using forensicGEM and the Liquid-Handling Workstation epMotion 5075 MC (PDF 292k)
Automated isolation of plant genomic DNA using ChargeSwitch® technology (PDF 148k)
Reproducible and Easy Automated Purification of Plant Genomic DNA (PDF 160k)
Isolation of high quality plasmid DNA using the Perfectprep Plasmid 96 Vac Direct Bind kit on the epMotion  5075 Vac workstation (PDF 276k)
Isolation of high quality BAC DNA using the Perfectprep BAC 96 kit on the epMotion  5075 Vac workstation (PDF 388k)
Purification of PCR products using the Perfectprep PCR Cleanup 96 kit on the epMotion  5075 Vac workstation (PDF 356k)
Precision and accuracy to the epMotion system (PDF 104k)
Contamination test for automated dispensing system - epMotion 5070 and 5075 (PDF 128k)
Minimization of remaining volumes in plates and tubes (PDF 64k)
Loading the Invitrogen E-Gels with the epMotion 5075 (PDF 228k)
Guideline for processing the RNeasy 96 BioRobot 8000 kit on the epMotion 5075 VAC workstation (PDF 448k)
Guidelines for processing the QIAamp DNA Blood BioRobot MDx kit on the epMotion 5075 VAC workstation (PDF 652k)
High-throughput RNA preparation using the QIAGEN RNeasy® 96 BioRobot® 8000 kit on the workstation epMotion 5075 (PDF 124k)
Liquid Handling Go to top
Pipettes
Liquid Handling No. 1—Using liquid handling systems in the laboratory (PDF 116k)
Using liquid handling systems in the laboratory (PDF 116k)
Comparison of pipetting characteristics of electronic and manual pipettes using ELISA (PDF 80k)
Photometric pipette test (PDF 36k)
Microarrays Go to top
DualChip™ microarray slides
DualChip microarray series tips and tricks (PDF 2.3M)
The Eppendorf DualChip analysis control system  (PDF 148k)
Gene expression profiling with DualChip microarrays (PDF 228k)
Xmer™ technology: probe selection for DualChip microarrays  (PDF 128k)
DualChip human aging for gene expression profiling of two human cell lines: MCR-5 and A43 (PDF 88k)
DualChip human apoptosis: expression profiling of Etoposide-induced Apoptosis (PDF 572k)
DualChip human cancer: for gene expression profiling of human tumors (PDF 404k)
DualChip human general: expression profiling on a low-density DNA microarray (PDF 124k)
DualChip human hepato: a reliable tool for gene expression analysis in toxicology (PDF 52k)
DualChip human RNAi side effects: determination of siRNA specificity through gene expression profiling (PDF 224k)
DualChip mouse cancer: gene expression profiling of the proto-oncogene PLAG1 (PDF 448k)
DualChip rat GPCR: analysis of rat G-protein-coupled receptor pathways on a low-density microarray (PDF 44k)
DualChip rat hepato: gene expression profiling of PB and PCN (PDF 64k)
PCR Go to top
Consumables
twin.tec plates
An assessment of Eppendorf twin.tec plate performance in PCR applications (PDF 96k)
Thermal Cyclers
General notes on primer design in PCR
Mastercycler ep gradient
Fast PCR – speed and precision with optimized systems (PDF 208k)
Mastercycler®
Single-copy virus detection by GenPoint in situ hybridisation on the Eppendorf Mastercycler (PDF 64k)
Optimizing DNA amplification protocols using the Eppendorf Mastercycler
Mastercycler gradient
Amplification of Epstein-Barr Virus Exon C with difficult primers using Eppendorf Mastercycler gradient (PDF 316k)
Two-dimensional optimization of a semi-nested PCR for detecting listeria monocytogenes (PDF 100k)
Gradient PCR
A better way to optimize: two-step gradient PCR (PDF 240k)
PCR optimization
Optimized dengue RT-PCR
Optimizing the direct amplification of missing cDNA 5' ends using the Mastercycler gradient (PDF 100k)
The use of the Mastercycler gradient for single-cell PCR and preimplantation diagnosis (PDF 112k)
qPCR
Thermal Cyclers
Mastercycler ep realplex – qPCR
Successful qPCR with small reaction volumes on Eppendorf Mastercycler ep realplex (PDF 644k)
The Mastercycler ep realplex – a flexible device for fast and accurate real-time PCR (PDF 648k)
Shakers/Mixers Go to top
Microarray-based gryA genotyping of quinolone resistance in E.coli using Thermomixer R (comfort) with slide adapter (PDF 164k)
Eppendorf MixMate – Experimental evidence of controlled mixing, using a PCR-based chessboard assay (PDF 252k)
Comparison of mixing performance in 96- and 384-well plates of Eppendorf MixMate and competitor devices (PDF 76k)
Normalizing cDNA libraries using the Eppendorf Thermomixer (PDF 508k)
The use of Thermomixer comfort for AIMS (PDF 336k)
BSE and Eppendorf (PDF 212k)
BSE: background, perspectives, centrifugation (PDF 504k)
Areas of application for ThermoStat plus (PDF 112k)
What about cell cultures? (PDF 84k)
Exchangeable thermoblocks for slides (PDF 112k)
Hot or cold! Shaken - not stirred (Mission possible hot and cold) (PDF 240k)
Userguide: Eppendorf Thermomixer comfort/compact, Eppendorf ThermoStat plus (PDF 128k)
Flyer: IVD (in vitro diagnostics) directive (PDF 140k)
Brochure: All for one, and one with all! The Thermomixer and ThermoStat plus product range (PDF 924k)
Vacuum Concentration Go to top
Using a Concentrator 5301 in a sequencing laboratory (PDF 668k)
A service of Brinkmann Instruments, (Canada) Ltd. Copyright © 2008. All rights reserved.