Search:
Applications
FAQs
Manuals
Literature Library
Metrohm Support
Get Support
Subscriptions
 
Enter your postal code:
 
Applications
Practical Information for the Molecular Biologist

Detection of Nucleic Acids and Proteins

Recommended Agarose Gel Percentages for Resolution of DNA

Gel percentage DNA size range
0.5% 1,000–30,000 bp
0.7% 800–12,000 bp
1.0% 500–10,000 bp
1.2% 400–7,000 bp
1.5% 200–3,000 bp
2.0% 50–2,000 bp

Recommended Polyacrylamide Gel Percentages for Resolution of DNA

Gel percentage DNA size range
3.5% 100–2,000 bp
5.0% 75–500 bp
8.0% 50–400 bp
12.0% 35–250 bp
15.0% 20–150 bp
20.0% 5–100 bp

Recommended Polyacrylamide Gel Percentages for Resolution of Proteins

Gel percentage Protein size range
8% 40–200 kDa
10% 21–100 kDa
12% 10–40 kDa

Dye Migration

Dye Migration in Polyacrylamide Non-denaturing Gels

Gel percentage Bromophenol Blue Xylene Cyanol
3.5% 100 bp 460 bp
5.0% 65 bp 260 bp
8.0% 45 bp 160 bp
12.0% 20 bp 70 bp
15.0% 15 bp 60 bp
20.0% 12 bp 45 bp

Dye Migration in Polyacrylamide Denaturing Gels

Gel percentage Bromophenol Blue Xylene Cyanol
5.0% 35 nucleotides 140 nucleotides
6.0% 26 nucleotides 106 nucleotides
8.0% 19 nucleotides 75 nucleotides
10.0% 12 nucleotides 55 nucleotides
20.0% 8 nucleotides 28 nucleotides
A service of Brinkmann Instruments (Canada) Corp. Copyright © 2009. All rights reserved.