|
Nucleic Acid Conversions
Conversion of Weight to Absolute Quantity
(mol)
| 1 µg of 1,000 bp DNA |
= 1.52 pmol |
= 9.1 x 1011 molecules |
| 1 µg of pUC18/19 DNA (2,686 bp) |
= 0.57 pmol |
= 3.4 x 1011
molecules |
| 1 µg of pBR322 DNA (4,361 bp) |
= 0.35 pmol |
= 2.1 x 1011
molecules |
| 1 µg of M13mp18/19 DNA (7,249 bp)
|
= 0.21 pmol |
= 1.3 x 1011
molecules |
| 1 µg of λ DNA (48,502 bp) |
= 0.30 pmol |
= 1.8 x 1010
molecules |
Conversion of Absolute Quantity (mol) to
Weight
| 1 pmol of 1,000 bp DNA |
= 0.66 µg |
| 1 pmol of pUC18/19 DNA (2,686 bp) |
= 1.77 µg |
| 1 pmol of pBR322 DNA (4,361 bp) |
= 2.88 µg |
| 1 pmol of M13mp18/19 DNA (7,249 bp) |
= 4.78 µg |
| 1 pmol of DNA (48,502 bp) |
= 32.01 µg |
| Molecular Weights for
Nucleotides
| Compound |
Molecular weight |
| AMP |
347.2 |
| CMP |
323.2 |
| GMP |
363.2 |
| UMP |
324.2 |
| dAMP |
331.2 |
| dCMP |
307.2 |
| dGMP |
347.2 |
| dTMP |
322.2 |
|
Molecular Weight of DNA Fragments
| 500 bp dsDNA |
= 325,000 Dalton |
| 500 nt (Nucleotide) ssDNA |
= 162,500 Dalton |
| 1 kb dsDNA |
= 660,000 Dalton |
| 1 kb ssDNA |
= 330,000 Dalton |
| 1 kb ssRNA |
= 340,000 Dalton |
| 1 MDa of dsDNA |
= 1.52 kb |
The average molecular weight of dNMP is 325
Dalton.
The average molecular weight of DNA base pair is 650 Dalton. |
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