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Applications
Practical Information for the Molecular Biologist

Radioisotopes

Units and Measurements of Radioactivity

The SI unit of measurement of radioactivity is the Becquerel: 1 Becquerel (Bq) = 1 Disintegration per second

The more commonly encountered units are disintegration per minute (dpm) and curie (Ci)

Conversion factors:    
1 Bq = 60 dpm  
1 Ci = 3.7 x 1010 Bq = 2.22 x 1012 dpm
1 millicurie (mCi) = 3.7 x 107 Bq = 2.22 x 109 dpm
1 microcurie (µCi) = 3.7 x 104 Bq = 2.22 x 106 dpm
1 day = 1.44 x 103 min. = 8.64 x 104 sec.
1 year = 5.26 x 105 min. = 3.16 x 107 sec.

counts per minute (cpm) x (counting efficiency)

Physical Properties of Commonly Used Radioisotopes

Nuclide Half-lifetime Emission Energy, max. (MeV) Range of emission, max. Rec. shielding (half value layer)
3H 12.43 years ß 0.0186 0.42 cm (air)
14C 5,370 years ß 0.156 21.8 cm (air)
32Pb 14.3 days ß 1.71 610 cm (air) Acrylic (1 cm)
        0.8 cm (water)
        0.76 cm (Plexiglas®)
33Pb 25.4 days ß 0.249 49 cm Acrylic (1 cm)
35S 87.4 days ß 0.167 24.4 cm (air)
125Ic 60 days γ 0.27–0.035 0.2 mm (lead) Lead (0.02 mm)

 

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